And we offer a wide variety of antibody labeling options for your subsequent experiments. Detection and Labelingĭetection methods, such as Western blot, ELISA, antibody purification test, and endotoxin detection, would be employed. Antibody purification is affinity purification method using Protein A/G resin. To polyclonal antibody, the serum would be harvest for antibody purification after test-bleed. To monoclonal antibody, the antibodies are produced via cells cultured in roller bottles. Screeningįor screening positive clones and long-term stability, parental cell lines can be subcloned through limiting-dilution cloning to obtain daughter cell lines. Cell FusionĪccording the ELISA result, spleen cells are harvested from two or three mice for hybridoma fusion. Please be noted, the host of custom antibody has a wide selections, such as mouse, rabbit, rat, chicken, goat, etc. At this stage, BIC usually take at least four rounds of immunization, until the ELISA titer of the last test-bleed is greater than 1:50,000. ImmunizationĪfter antigen preparation, five balb/c mice or two New Zealand rabbits are immunized with multiple rounds of immunization and test-bled (ELISA test is employed). With years of experience in exploring protein field, BIC would analyze and design antigen, for producing high quality antibodies, even for those difficult ones. With in-house antigen design tools, our scientists can rapidly and accurately analyze the protein sequence and predict which regions are immunogenic, unique and exposed. Antibody production generally starts from antigen analysis and design. Monoclonal and polyclonal antibody production has the similar process, except polyclonal antibody does not require cell fusion and screening. Using classical protocols that have been proven most effective by ourselves, the antibody production in BIC can ensure the quality and save your precious time and money. The Process of Antibody Production In BIC Not ideal for the most peptide antigens.Suitable for polyclonal antibody production.Good for large volume polyclonal antibodies. Good for small-volume polyclonal antibody projects.Not ideal for highly conserved mammalian proteins.High response to peptide and most protein antigens.Good host species for antibody production.Not suitable for short peptide antigens.Common host species for antibody generation.The following table provides advantages and disadvantages of each host species. Lot-to-lot variability in characterization.Quantity available limited by the number of bleeds Prone to produce non-specific antibodies, and produce background signals in some applications.High requirement for the purity of immunogen.Multiple host species, including non-mammalian hosts.One antibody can work for a variety of applications.Request a Quote Now! Custom Antibody Production Categories Further antibody services are available, such as antibody labeling, sequencing, recombinant production, etc.Feel free to contact us for any questions. Please remember our guarantee: Using protein antigen, ELISA titer of the antibody produced by BIC is greater than 1: 128,000. With the extensive experience in hybridoma, CHO, and HEK-293 cells, our custom antibody production service does not only offer you antibodies, but provides the complete support for all steps in antibody production. After more than 3,000 successful projects, we are proud of ourselves in being able to provide high quality antibodies with affordable price. (BIC) was founded by a group of skilled scientists who have specialized in antibody production for decades.
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